Highly sensitive biotin-labelled hybridization probe.

Detection of Hepatitis B Virus DNA in Human Serum by Dot Hybridization Using a Biotin-Labelled Probe*

Radiationless deactivation of hybridization-sensitive DNA probe

In this study we attempt to determine the deactivation mechanism of a hybridization-sensitive DNA probe by using steady state absorption and fluorescence spectroscopy. Thiazole orange or 2,20Methylenebis(N-alkylbenzothiazole) is covalently linked to a single nucleotide in the DNA probe. Radiationless deactivation of the fluorophores is mainly determined by photo-induced isomerization of the met...

Hybridization-sensitive fluorescent DNA probe with self-avoidance ability.

Hybridization-sensitive fluorescent probes have an inherent disadvantage: self-dimerization of the probe prevents the fluorescence quenching prior to hybridization with the target, resulting in a high background signal. To avoid self-dimerization of probes, we focused on a base pair formed by 2'-deoxyinosine (I) and N(4)-ethyl-2'-deoxycytidine (E). I and E bases form more stable base pairs with...

Preparation of Biotin-labelled Toxoplasma Antigen

A method for the simple preparation ofbiotin-labelled toxoplasma antigen was used with avidin peroxidase in an IgM-capture enzyme linked immunosorbent assay (BAM-ELISA). Although the overall predictive value ofa positive result was only 38%, its low-cost and 100% sensitivity makes it a very suitable screening test. Positive results can be confirmed by an alternative assay, thus providing a more...

The preparation of biotin-labelled hyaluronidase

Centre and purified using the appropriate Synsorbs (Chembiomed Ltd., Alberta, Canada). Monoclonal anti-A was isolated in the M.R.C. Human Biochemical Genetics Unit. In one experiment lactase from two individuals of different blood group (A and 0) was purified by immunoaffinity chromatography as described in Potter et al. (1985). Using the A blood-group specific antibodies and the lectin from He...